Among the following, which is the most frequently utilized automated method for glucose determination?

The most frequently utilized automated method for glucose determination is the coupled enzyme system consisting of glucose oxidase and peroxidase. This method is widely used in clinical laboratories due to its specificity and sensitivity for glucose measurement.

In this system, glucose is oxidized by glucose oxidase, producing hydrogen peroxide as a by-product. This hydrogen peroxide is then reacted with peroxidase and a color reagent, leading to a measurable color change. The intensity of the color is proportional to the glucose concentration and can be quantitatively measured using photometric techniques. This makes the method suitable for high-throughput automated systems, allowing for rapid and reliable glucose testing.

The hexokinase method, while also accurate, is less frequently used in automated systems compared to the coupled enzyme method. It involves the reaction of glucose and ATP to form glucose-6-phosphate and ADP, which requires additional steps and reagents that can complicate automation.

Gas chromatography, while an effective technique for analyzing volatile substances, is not typically employed for routine glucose determination in clinical settings. This method is more suited for research or specialized testing environments.

The colorimetric glucose assay, though a fundamental method, often lacks the sensitivity and specificity of modern automated techniques like the coupled enzyme system. It also