How can hemolysis be prevented during blood sample collection?

Preventing hemolysis during blood sample collection is crucial for obtaining accurate test results. The correct approach involves avoiding vigorous twisting of the sample tube. When blood is drawn, proper tube handling is essential. If the tube is twisted too vigorously, it can shear red blood cells, leading to hemolysis. Maintaining a gentle technique when mixing or inverting the tube after collection helps preserve the integrity of the blood cells, thereby preventing hemolysis.

On the other hand, using a larger needle, while it may reduce the risk of hemolysis to some extent by decreasing the chance of cell lysis from high pressure, it does not specifically address the handling of the sample post-collection. Collecting samples in warmer temperatures does not directly relate to preventing hemolysis; temperature effects on blood cellular components are minimal during the brief window of sample collection. Minimizing pressure on the blood vessel, although generally a good practice, primarily serves to avoid hematoma formation rather than directly preventing hemolysis during the sample collection process. Therefore, avoiding vigorous twisting of the sample tube is the most effective method to prevent cell damage and maintain sample integrity.