Which of the following enzymes is most greatly affected by hemolysis?

Lactate dehydrogenase (LDH) is the enzyme that is most greatly affected by hemolysis. When red blood cells (RBCs) are damaged or lysed, LDH is released from the intracellular environment into the serum, leading to elevated levels. This is why LDH is often used as a marker for tissue damage and hemolysis.

The other enzymes listed do not experience as significant an increase following hemolysis. Alanine aminotransferase (ALT) and gamma-glutamyltransferase (GGT) are primarily associated with liver function, and while they can also be released from damaged liver cells, they are not as sensitive to hemolysis as LDH. Amylase, on the other hand, is related to pancreatic function and does not show significant changes due to hemolysis. Therefore, LDH's sensitivity to hemolysis makes it the most affected among the listed enzymes.