Which of the following methods is considered the reference method for measuring glucose due to its increased accuracy and sensitivity?

The hexokinase method is recognized as the reference method for measuring glucose due to its high accuracy and sensitivity. This method employs the enzyme hexokinase, which catalyzes the phosphorylation of glucose to glucose-6-phosphate in the presence of ATP. The amount of glucose is determined by measuring the amount of NADPH produced through a coupled reaction involving glucose-6-phosphate dehydrogenase.

One of the key advantages of the hexokinase method lies in its ability to reduce interference from other sugars and substances that can affect glucose measurements, making it a reliable choice for clinical settings. Its specificity and reliability render it superior to alternative methods, ensuring precise glucose levels are reported, which is crucial for patient management, especially in diabetes care. This heightened accuracy forms the basis for its status as a reference method against which other glucose measurement techniques are often compared.

In contrast, other methods may not provide the same level of precision or may be more susceptible to interference, thus lessening their reliability in comparison to the hexokinase approach.