Which type of cuvette is necessary for measurements in the ultraviolet range of a spectrophotometer?

In spectrophotometry, the choice of cuvette material is critical, particularly when measuring absorbance in the ultraviolet (UV) range of the spectrum. Quartz cuvettes are specifically designed for this purpose because they have excellent transmission properties in the UV region, typically from about 200 nm to 400 nm.

Unlike glass and plastic, which can absorb UV light and may alter the readings, quartz allows for accurate measurement of UV wavelengths without interference. This transparency is essential for obtaining reliable and precise absorbance data in biochemical analyses. Metal cuvettes, on the other hand, are not suitable either, as they reflect light and do not permit transmission for spectrophotometric measurements.

Therefore, the need for a cuvette material that can handle the specific wavelengths of UV light while providing minimal absorption makes quartz the appropriate choice for such measurements.