Which classical method is used for the detection of creatinine in the laboratory?

Which classical method is used for the detection of creatinine in the laboratory?

• A. Enzymatic method
• B. Jaffe reaction
• C. Colorimetric assay
• D. HPLC analysis

Answer: (B)

The Jaffe reaction is a classical method used for the detection of creatinine in the laboratory due to its established biochemical mechanism. This method relies on the reaction between creatinine and alkaline picrate, resulting in a colored complex that can be quantitatively measured. In a typical laboratory setting, the intensity of the color produced is directly proportional to the concentration of creatinine in the sample being analyzed, making it a practical and reliable method for routine clinical use. This reaction is advantageous because it is relatively simple and cost-effective compared to more modern techniques. It has a long history in clinical laboratories and allows for a rapid assessment of kidney function through creatinine clearance measurements. Although other methods like enzymatic assays, colorimetric assays, and HPLC can also be used for creatinine measurement, the Jaffe reaction remains a widely recognized and traditional approach in clinical laboratory diagnostics.

The Jaffe reaction is a classical method used for the detection of creatinine in the laboratory due to its established biochemical mechanism. This method relies on the reaction between creatinine and alkaline picrate, resulting in a colored complex that can be quantitatively measured. In a typical laboratory setting, the intensity of the color produced is directly proportional to the concentration of creatinine in the sample being analyzed, making it a practical and reliable method for routine clinical use.

This reaction is advantageous because it is relatively simple and cost-effective compared to more modern techniques. It has a long history in clinical laboratories and allows for a rapid assessment of kidney function through creatinine clearance measurements. Although other methods like enzymatic assays, colorimetric assays, and HPLC can also be used for creatinine measurement, the Jaffe reaction remains a widely recognized and traditional approach in clinical laboratory diagnostics.