Which of the following is the preferred reagent for measuring albumin by the dye binding method due to less interference?

The preferred reagent for measuring albumin by the dye binding method is bromcresol purple. This reagent is specifically chosen because it exhibits a greater specificity for albumin and is less prone to interference from other proteins and substances present in the sample.

Bromcresol purple's pH sensitivity allows it to bind effectively with albumin, forming a stable complex that can be measured photometrically. This means that the results are more reliable, as they are less affected by common interferents that might alter the absorbance readings.

In contrast, bromcresol green, while commonly used, can have issues with interference from other plasma proteins such as globulins, which can lead to inaccurate readings. Similarly, HABA (4-hydroxyazobenzene-2-carboxylic acid) is also used for measuring proteins but is not as selective for albumin compared to bromcresol purple. Methyl orange, on the other hand, is not typically used for this purpose as it does not have the appropriate binding characteristics for albumin.

This specificity and reduced susceptibility to interference make bromcresol purple the superior choice among the available options for the accurate measurement of albumin in clinical laboratory settings.