Which reagent is used in the alkaline oxidation of uric acid?

Phosphotungstic acid is the reagent used in the alkaline oxidation of uric acid. This reagent acts as an effective oxidizing agent in a basic solution, facilitating the conversion of uric acid into a measurable form. The oxidation process is significant in several biochemical analyses since uric acid is a waste product derived from purine metabolism.

When phosphotungstic acid is employed in the assay, it helps to ensure accurate quantification of uric acid levels in biological samples. Moreover, this method can help assess various clinical conditions, including gout and other disorders associated with purine metabolism, by enabling precise monitoring of uric acid levels. The choice of phosphotungstic acid specifically is important due to its stability and effectiveness at alkaline pH, making it ideal for this reaction.

Other acids listed, such as phosphoric acid, sulfuric acid, and acetic acid, do not serve the role of oxidizing agents in the alkaline oxidation of uric acid. Instead, they have different applications in biochemical contexts and do not provide the oxidation properties necessary for this specific reaction.